Next Article in Journal
Cistus incanus from Strandja Mountain as a Source of Bioactive Antioxidants
Next Article in Special Issue
Imaging and Spectroscopy of Natural Fluorophores in Pine Needles
Previous Article in Journal
Deciphering the Evolution and Development of the Cuticle by Studying Lipid Transfer Proteins in Mosses and Liverworts
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle

Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus

1
Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany
2
School of Analytical Sciences Adlershof SALSA, Humboldt-Universität zu Berlin, Albert-Einstein-Str. 5-9, 12489 Berlin, Germany
3
Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Lentzeallee 55/57, 14195 Berlin, Germany
4
BAM Federal Institute for Materials Research and Testing, Richard-Willstatter-Straße 11, 12489 Berlin, Germany
*
Author to whom correspondence should be addressed.
Received: 27 December 2017 / Revised: 19 January 2018 / Accepted: 23 January 2018 / Published: 25 January 2018
Full-Text   |   PDF [2987 KB, uploaded 25 January 2018]   |  

Abstract

Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues. View Full-Text
Keywords: Cucumis sativus; cell wall; xylem; Raman spectroscopy; chemical imaging; cellulose; lignin; principal component analysis; hierarchical cluster analysis; carotene Cucumis sativus; cell wall; xylem; Raman spectroscopy; chemical imaging; cellulose; lignin; principal component analysis; hierarchical cluster analysis; carotene
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Zeise, I.; Heiner, Z.; Holz, S.; Joester, M.; Büttner, C.; Kneipp, J. Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus. Plants 2018, 7, 7.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top