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J. Dev. Biol. 2018, 6(2), 9; https://doi.org/10.3390/jdb6020009

Requirement of the Dynein-Adaptor Spindly for Mitotic and Post-Mitotic Functions in Drosophila

1
Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
2
Institut für Biologie, Fachgebiet Entwicklungsgenetik, Universität Kassel, Heinrich-Plett Str. 40, 34321 Kassel, Germany
3
Centre of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
Present Address: Nikon Instruments Incorporated, 1300 Walt Whitman Rd., Melville, NY 11747, USA.
*
Author to whom correspondence should be addressed.
Received: 9 January 2018 / Revised: 20 March 2018 / Accepted: 27 March 2018 / Published: 30 March 2018
(This article belongs to the Special Issue Drosophila - A Model System for Developmental Biology)
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Abstract

Spindly was originally identified as a specific regulator of Dynein activity at the kinetochore. In early prometaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here, we present loss- and gain-of-function studies of Spindly using transgenic RNAi in Drosophila. Knock-down of Spindly in the female germ line results in mitotic arrest during embryonic cleavage divisions. We investigated the requirements of Spindly protein domains for its localisation and function, and found that the carboxy-terminal region controls Spindly localisation in a cell-type specific manner. Overexpression of Spindly in the female germ line is embryonic lethal and results in altered egg morphology. To determine whether Spindly plays a role in post-mitotic cells, we altered Spindly protein levels in migrating cells and found that ovarian border cell migration is sensitive to the levels of Spindly protein. Our study uncovers novel functions of Spindly and a differential, functional requirement for its carboxy-terminal region in Drosophila. View Full-Text
Keywords: Drosophila; mitosis; cell migration; mitotic spindle; Dynein Drosophila; mitosis; cell migration; mitotic spindle; Dynein
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Clemente, G.D.; Hannaford, M.R.; Beati, H.; Kapp, K.; Januschke, J.; Griffis, E.R.; Müller, H.-A.J. Requirement of the Dynein-Adaptor Spindly for Mitotic and Post-Mitotic Functions in Drosophila. J. Dev. Biol. 2018, 6, 9.

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