Next Article in Journal
Restricted Pax3 Deletion within the Neural Tube Results in Congenital Hydrocephalus
Previous Article in Journal
Acknowledgement to Reviewers of Journal of Developmental Biology in 2015
Article Menu

Export Article

Open AccessArticle
J. Dev. Biol. 2016, 4(1), 6; doi:10.3390/jdb4010006

Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis

Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, 123182 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Andy Wessels
Received: 16 December 2015 / Revised: 18 January 2016 / Accepted: 20 January 2016 / Published: 27 January 2016
View Full-Text   |   Download PDF [1854 KB, uploaded 27 January 2016]   |  

Abstract

Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study. View Full-Text
Keywords: DNA polymerase iota; loach; DNA synthesis; DNA repair DNA polymerase iota; loach; DNA synthesis; DNA repair
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gening, L.V.; Lakhin, A.V.; Makarova, I.V.; Nenasheva, V.V.; Andreeva, L.E.; Tarantul, V.Z. Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis. J. Dev. Biol. 2016, 4, 6.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Dev. Biol. EISSN 2221-3759 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top