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Biomolecules 2018, 8(3), 48; https://doi.org/10.3390/biom8030048

A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells

Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA
These authors contributed equally to this work.
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Received: 1 May 2018 / Revised: 22 June 2018 / Accepted: 25 June 2018 / Published: 9 July 2018
(This article belongs to the Special Issue Cell Penetrating Peptides)
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Abstract

Retinoic acid (RA) is a bioactive lipid that has been shown to promote neural stem cell differentiation. However, the highly hydrophobic molecule needs to first solubilize and translocate across the cell membrane in order to exert a biological response. The cell entry of RA can be aided by cell penetrating peptides (CPPs), which are short amino acid sequences that are able to carry bioactive cargo past the cell membrane. In this work, a novel cell penetrating peptide was developed to deliver RA to human neural stem cells and, subsequently, promote neuronal differentiation. The novel CPP consists of a repeating sequence, whose number of repeats is proportional to the efficiency of cell penetration. Using fluorescence microscopy, the mode of translocation was determined to be related to an endocytic pathway. The levels of β-III tubulin (Tubb3) and microtubule associated protein 2 (MAP2) expression in neural stem cells treated with RA conjugated to the CPP were assessed by quantitative immunocytochemistry. View Full-Text
Keywords: cell penetrating peptide; neural stem cell; retinoic acid; differentiation; cytotoxicity; endocytosis cell penetrating peptide; neural stem cell; retinoic acid; differentiation; cytotoxicity; endocytosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ma, W.; Jin, G.-W.; Gehret, P.M.; Chada, N.C.; Suh, W.H. A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells. Biomolecules 2018, 8, 48.

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