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Biomolecules 2017, 7(2), 36; doi:10.3390/biom7020036

QueF-Like, a Non-Homologous Archaeosine Synthase from the Crenarchaeota

1
Department of Chemistry, Portland State University, Portland, OR 97207, USA
2
The Department of Microbiology and Cell Science Department, University of Florida, Gainesville, FL 32611, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jürg Bähler
Received: 28 February 2017 / Revised: 23 March 2017 / Accepted: 24 March 2017 / Published: 6 April 2017
(This article belongs to the Collection RNA Modifications)
View Full-Text   |   Download PDF [2333 KB, uploaded 6 April 2017]   |  

Abstract

Archaeosine (G+) is a structurally complex modified nucleoside ubiquitous to the Archaea, where it is found in the D-loop of virtually all archaeal transfer RNA (tRNA). Its unique structure, which includes a formamidine group that carries a formal positive charge, and location in the tRNA, led to the proposal that it serves a key role in stabilizing tRNA structure. Although G+ is limited to the Archaea, it is structurally related to the bacterial modified nucleoside queuosine, and the two share homologous enzymes for the early steps of their biosynthesis. In the Euryarchaeota, the last step of the archaeosine biosynthetic pathway involves the amidation of a nitrile group on an archaeosine precursor to give formamidine, a reaction catalyzed by the enzyme Archaeosine Synthase (ArcS). Most Crenarchaeota lack ArcS, but possess two proteins that inversely distribute with ArcS and each other, and are implicated in G+ biosynthesis. Here, we describe biochemical studies of one of these, the protein QueF-like (QueF-L) from Pyrobaculum calidifontis, that demonstrate the catalytic activity of QueF-L, establish where in the pathway QueF-L acts, and identify the source of ammonia in the reaction. View Full-Text
Keywords: archaeosine; biosynthesis; modified nucleoside; tunneling-fold superfamily; amidinotransferase archaeosine; biosynthesis; modified nucleoside; tunneling-fold superfamily; amidinotransferase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bon Ramos, A.; Bao, L.; Turner, B.; de Crécy-Lagard, V.; Iwata-Reuyl, D. QueF-Like, a Non-Homologous Archaeosine Synthase from the Crenarchaeota. Biomolecules 2017, 7, 36.

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