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Biomolecules 2015, 5(4), 3029-3050; doi:10.3390/biom5043029

RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences

1,2,†
,
1,†
and
1,2,*
1
Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands
2
Department of Biomolecular Chemistry, Radboud Institute for Molecular Life Sciences, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Denis Drainas
Received: 29 July 2015 / Revised: 16 October 2015 / Accepted: 29 October 2015 / Published: 9 November 2015
(This article belongs to the Special Issue Function and Structure of RNase P in Fungi, Bacteria and Human Cells)
View Full-Text   |   Download PDF [439 KB, uploaded 9 November 2015]   |  

Abstract

The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels. View Full-Text
Keywords: external guide sequence; RNase P; RNA cleavage; RNA knockdown; RNA targeting external guide sequence; RNase P; RNA cleavage; RNA knockdown; RNA targeting
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Derksen, M.; Mertens, V.; Pruijn, G.J. RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences. Biomolecules 2015, 5, 3029-3050.

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