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Biomolecules 2015, 5(2), 647-667; doi:10.3390/biom5020647

Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases

1
Committee on Genetics, Genomics, and Systems Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
2
Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Sivaramesh Wigneshweraraj and Deborah M. Hinton
Received: 20 February 2015 / Revised: 1 April 2015 / Accepted: 13 April 2015 / Published: 27 April 2015
(This article belongs to the Special Issue Bacterial RNA Polymerase)
View Full-Text   |   Download PDF [2305 KB, uploaded 7 May 2015]   |  

Abstract

Bacteriophage N4 regulates the temporal expression of its genome through the activity of three distinct RNA polymerases (RNAP). Expression of the early genes is carried out by a phage-encoded, virion-encapsidated RNAP (vRNAP) that is injected into the host at the onset of infection and transcribes the early genes. These encode the components of new transcriptional machinery (N4 RNAPII and cofactors) responsible for the synthesis of middle RNAs. Both N4 RNAPs belong to the T7-like “single-subunit” family of polymerases. Herein, we describe their mechanisms of promoter recognition, regulation, and roles in the phage life cycle. View Full-Text
Keywords: bacteriophage N4; single-subunit polymerases; N4 virion RNA polymerase; N4 RNAPII bacteriophage N4; single-subunit polymerases; N4 virion RNA polymerase; N4 RNAPII
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lenneman, B.R.; Rothman-Denes, L.B. Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases. Biomolecules 2015, 5, 647-667.

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