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Production of Fungal Glucoamylase for Glucose Production from Food Waste
School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China
* Author to whom correspondence should be addressed.
Received: 6 August 2013; in revised form: 15 September 2013 / Accepted: 17 September 2013 / Published: 19 September 2013
Abstract: The feasibility of using pastry waste as resource for glucoamylase (GA) production via solid state fermentation (SSF) was studied. The crude GA extract obtained was used for glucose production from mixed food waste. Our results showed that pastry waste could be used as a sole substrate for GA production. A maximal GA activity of 76.1 ± 6.1 U/mL was obtained at Day 10. The optimal pH and reaction temperature for the crude GA extract for hydrolysis were pH 5.5 and 55 °C, respectively. Under this condition, the half-life of the GA extract was 315.0 minutes with a deactivation constant (kd) 2.20 × 10−3minutes−1. The application of the crude GA extract for mixed food waste hydrolysis and glucose production was successfully demonstrated. Approximately 53 g glucose was recovered from 100 g of mixed food waste in 1 h under the optimal digestion conditions, highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of glucose applicable as carbon source in many biotechnological processes.
Keywords: glucoamylase production; Aspergillus awamori; food waste hydrolysis; glucose production
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Lam, W.C.; Pleissner, D.; Lin, C.S.K. Production of Fungal Glucoamylase for Glucose Production from Food Waste. Biomolecules 2013, 3, 651-661.
Lam WC, Pleissner D, Lin CSK. Production of Fungal Glucoamylase for Glucose Production from Food Waste. Biomolecules. 2013; 3(3):651-661.
Lam, Wan C.; Pleissner, Daniel; Lin, Carol S.K. 2013. "Production of Fungal Glucoamylase for Glucose Production from Food Waste." Biomolecules 3, no. 3: 651-661.