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Metabolites 2017, 7(4), 57; https://doi.org/10.3390/metabo7040057

Metabolic Profile of the Cellulolytic Industrial Actinomycete Thermobifida fusca

1
VCU Life Sciences, Virginia Commonwealth University, Richmond 23284, VA, USA
2
Chemical and Life Sciences Engineering, Virginia Commonwealth University, Richmond 23284, VA, USA
*
Author to whom correspondence should be addressed.
Received: 30 September 2017 / Revised: 3 November 2017 / Accepted: 8 November 2017 / Published: 11 November 2017
(This article belongs to the Special Issue Metabolic Network Models Volume 2)
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Abstract

Actinomycetes have a long history of being the source of numerous valuable natural products and medicinals. To expedite product discovery and optimization of biochemical production, high-throughput technologies can now be used to screen the library of compounds present (or produced) at a given time in an organism. This not only facilitates chemical product screening, but also provides a comprehensive methodology to the study cellular metabolic networks to inform cellular engineering. Here, we present some of the first metabolomic data of the industrial cellulolytic actinomycete Thermobifida fusca generated using LC-MS/MS. The underlying objective of conducting global metabolite profiling was to gain better insight on the innate capabilities of T. fusca, with a long-term goal of facilitating T. fusca-based bioprocesses. The T. fusca metabolome was characterized for growth on two cellulose-relevant carbon sources, cellobiose and Avicel. Furthermore, the comprehensive list of measured metabolites was computationally integrated into a metabolic model of T. fusca, to study metabolic shifts in the network flux associated with carbohydrate and amino acid metabolism. View Full-Text
Keywords: metabolomics; metabolites; metabolic model; actinomycetes; cellobiose; avicel metabolomics; metabolites; metabolic model; actinomycetes; cellobiose; avicel
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Vanee, N.; Brooks, J.P.; Fong, S.S. Metabolic Profile of the Cellulolytic Industrial Actinomycete Thermobifida fusca. Metabolites 2017, 7, 57.

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