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Metabolites 2017, 7(4), 50; https://doi.org/10.3390/metabo7040050

Exometabolomic Analysis of Cross-Feeding Metabolites

Lawrence Berkeley National Laboratory, Cyclotron Road, Berkeley 94720, CA, USA
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Received: 12 September 2017 / Revised: 1 October 2017 / Accepted: 2 October 2017 / Published: 4 October 2017
(This article belongs to the Special Issue Environmental Metabolomics)
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Abstract

Microbial consortia have the potential to perform complex, industrially important tasks. The design of microbial consortia requires knowledge of the substrate preferences and metabolic outputs of each member, to allow understanding of potential interactions such as competition and beneficial metabolic exchange. Here, we used exometabolite profiling to follow the resource processing by a microbial co-culture of two biotechnologically relevant microbes, the bacterial cellulose degrader Cellulomonas fimi, and the oleaginous yeast Yarrowia lipolytica. We characterized the substrate preferences of the two strains on compounds typically found in lignocellulose hydrolysates. This allowed prediction that specific sugars resulting from hemicellulose polysaccharide degradation by C. fimi may serve as a cross-feeding metabolites to Y. lipolytica in co-culture. We also showed that products of ionic liquid-treated switchgrass lignocellulose degradation by C. fimi were channeled to Y. lipolytica in a co-culture. Additionally, we observed metabolites, such as shikimic acid accumulating in the co-culture supernatants, suggesting the potential for producing interesting co-products. Insights gained from characterizing the exometabolite profiles of individual and co-cultures of the two strains can help to refine this interaction, and guide strategies for making this an industrially viable co-culture to produce valuable products from lignocellulose material. View Full-Text
Keywords: exometabolomics; bioprocessing; microbial consortia; cross-feeding; Yarrowia lipolytica; Cellulomonas fimi; lignocellulose exometabolomics; bioprocessing; microbial consortia; cross-feeding; Yarrowia lipolytica; Cellulomonas fimi; lignocellulose
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Lubbe, A.; Bowen, B.P.; Northen, T. Exometabolomic Analysis of Cross-Feeding Metabolites. Metabolites 2017, 7, 50.

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