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Metabolites 2015, 5(4), 794-813; doi:10.3390/metabo5040794

Flux Balance Analysis Inspired Bioprocess Upgrading for Lycopene Production by a Metabolically Engineered Strain of Yarrowia lipolytica

1
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
2
INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France
3
Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), 130 Meilong Road, Shanghai 200237, China
These authors contributed equally to this work.
Present address: INRA, UMR782 GMPA, AgroParisTech Campus, CBAI, F-78850 Thiverval-Grignon, France
*
Author to whom correspondence should be addressed.
Academic Editors: Kazuyuki Shimizu and Peter Meikle
Received: 29 August 2015 / Revised: 1 December 2015 / Accepted: 10 December 2015 / Published: 21 December 2015
(This article belongs to the Special Issue Metabolic Flux Analysis)
View Full-Text   |   Download PDF [318 KB, uploaded 22 December 2015]   |  

Abstract

Genome-scale metabolic models embody a significant advantage of systems biology since their applications as metabolic flux simulation models enable predictions for the production of industrially-interesting metabolites. The biotechnological production of lycopene from Yarrowia lipolytica is an emerging scope that has not been fully scrutinized, especially for what concerns cultivation conditions of newly generated engineered strains. In this study, by combining flux balance analysis (FBA) and Plackett-Burman design, we screened chemicals for lycopene production from a metabolically engineered strain of Y. lipolytica. Lycopene concentrations of 126 and 242 mg/L were achieved correspondingly from the FBA-independent and the FBA-assisted designed media in fed-batch cultivation mode. Transcriptional studies revealed upregulations of heterologous genes in media designed according to FBA, thus implying the efficiency of model predictions. Our study will potentially support upgraded lycopene and other terpenoids production from existing or prospect bioengineered strains of Y. lipolytica and/or closely related yeast species. View Full-Text
Keywords: Yarrowia lipolytica; lycopene; flux balance analysis; fermentation; metabolic network model yli v1.7 Yarrowia lipolytica; lycopene; flux balance analysis; fermentation; metabolic network model yli v1.7
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nambou, K.; Jian, X.; Zhang, X.; Wei, L.; Lou, J.; Madzak, C.; Hua, Q. Flux Balance Analysis Inspired Bioprocess Upgrading for Lycopene Production by a Metabolically Engineered Strain of Yarrowia lipolytica. Metabolites 2015, 5, 794-813.

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