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Physiological and Molecular Timing of the Glucose to Acetate Transition in Escherichia coli
Université de Toulouse; INSA, UPS, INP, 135 Avenue de Rangueil, Toulouse F-31077, France
INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, LISBP, Toulouse F-31400, France
CNRS, UMR5504, Toulouse F-31400, France
* Author to whom correspondence should be addressed.
Received: 7 June 2013; in revised form: 28 August 2013 / Accepted: 4 September 2013 / Published: 20 September 2013
Abstract: The glucose-acetate transition in Escherichia coli is a classical model of metabolic adaptation. Here, we describe the dynamics of the molecular processes involved in this metabolic transition, with a particular focus on glucose exhaustion. Although changes in the metabolome were observed before glucose exhaustion, our results point to a massive reshuffling at both the transcriptome and metabolome levels in the very first min following glucose exhaustion. A new transcriptional pattern, involving a change in genome expression in one-sixth of the E. coli genome, was established within 10 min and remained stable until the acetate was completely consumed. Changes in the metabolome took longer and stabilized 40 min after glucose exhaustion. Integration of multi-omics data revealed different modifications and timescales between the transcriptome and metabolome, but both point to a rapid adaptation of less than an hour. This work provides detailed information on the order, timing and extent of the molecular and physiological events that occur during the glucose-acetate transition and that are of particular interest for the development of dynamic models of metabolism.
Keywords: E. coli; carbon metabolism; transition; metabolome; transcriptome
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Enjalbert, B.; Letisse, F.; Portais, J.-C. Physiological and Molecular Timing of the Glucose to Acetate Transition in Escherichia coli. Metabolites 2013, 3, 820-837.
Enjalbert B, Letisse F, Portais J-C. Physiological and Molecular Timing of the Glucose to Acetate Transition in Escherichia coli. Metabolites. 2013; 3(3):820-837.
Enjalbert, Brice; Letisse, Fabien; Portais, Jean-Charles. 2013. "Physiological and Molecular Timing of the Glucose to Acetate Transition in Escherichia coli." Metabolites 3, no. 3: 820-837.