Metabolites 2013, 3(3), 701-717; doi:10.3390/metabo3030701
Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats
1
Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA
2
Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA
3
Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA
4
Department of Statistics, Oregon State University, Corvallis, OR 97331, USA
5
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA
*
Author to whom correspondence should be addressed.
Received: 3 June 2013 / Revised: 1 August 2013 / Accepted: 7 August 2013 / Published: 13 August 2013
(This article belongs to the Special Issue Response to Environment and Stress Metabolism)
Abstract
This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF) mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE) and LC-ion mobility spectrometry (IMS)-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS) instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p < 0.05 and fold change (FC) threshold > 1.5) features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes. View Full-Text
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Wickramasekara, S.I.; Zandkarimi, F.; Morré, J.; Kirkwood, J.; Legette, L.; Jiang, Y.; Gombart, A.F.; Stevens, J.F.; Maier, C.S. Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats. Metabolites 2013, 3, 701-717.
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