Metabolites 2011, 1(1), 21-40; doi:10.3390/metabo1010021
Review

Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics

Kui Yang 1 email and Xianlin Han 2,* email
1 Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA 2 Sanford-Burnham Medical Research Institute, Orlando, FL 32827, USA
* Author to whom correspondence should be addressed.
Received: 8 October 2011; in revised form: 2 November 2011 / Accepted: 4 November 2011 / Published: 11 November 2011
(This article belongs to the Special Issue Lipidomics)
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Abstract: Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.
Keywords: electrospray ionization mass spectrometry; lipidomics; quantification; shotgun lipidomics

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Cite This Article

MDPI and ACS Style

Yang, K.; Han, X. Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics. Metabolites 2011, 1, 21-40.

AMA Style

Yang K, Han X. Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics. Metabolites. 2011; 1(1):21-40.

Chicago/Turabian Style

Yang, Kui; Han, Xianlin. 2011. "Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics." Metabolites 1, no. 1: 21-40.

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