A PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran
AbstractStrongyloides stercoralis is a nematode causing serious infections in immunocompromised patients. In chronically infected patients, the low parasitic content as well as the resemblance of the larvae to several other species make diagnosis basedonmorphology difficult. In the present study, a PCR-based method targeting the internal transcribed sequence 2 (ITS2) of the rDNA region was examined for the molecular detection of S. stercoralis infection from the stool samples. A total of 1800 patients were included. Three fresh stool samples were collected per patient, and S. stercoralis isolates were identified by the morphological method. A subset of isolates was later used in the PCR-based method as positive controls. Additionally, negative and no-template controls were included. Data analysis was accomplished using an x2 test. Ap-value less than 0.05 was considered significant. In total, fivestool samples were found to be infected with S. stercoralis using the morphology method. PCR method detected S. stercoralis DNA target from all of the fiveDNA samples extracted from positive fecal samples. Conclusions: The PCR method used for amplifying a short fragment was successful for diagnosis of S. stercoralis in fecal samples and can be reliable for directly detecting the parasite bypassing morphological method. View Full-Text
Share & Cite This Article
Ghasemikhah, R.; Tabatabaiefar, M.A.; Shariatzadeh, S.A.; Shahbazi, A.; Hazratian, T. A PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran. Sci. Pharm. 2017, 85, 17.
Ghasemikhah R, Tabatabaiefar MA, Shariatzadeh SA, Shahbazi A, Hazratian T. A PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran. Scientia Pharmaceutica. 2017; 85(2):17.Chicago/Turabian Style
Ghasemikhah, Reza; Tabatabaiefar, Mohammad A.; Shariatzadeh, Seyed A.; Shahbazi, Abbas; Hazratian, Teymour. 2017. "A PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran." Sci. Pharm. 85, no. 2: 17.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.