Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes
AbstractLaggera alata extract (LAE) was quantitatively analyzed, and its principle components isochlorogenic acids were isolated and authenticated. Protective properties of LAE were studied using a D-galactosamine (D-GalN)-induced injury model in neonatal rat hepatocytes and a D-GalN-induced acute liver damage model in mice. Meanwhile, the effect of isochlorogenic acids derived from LAE on D-GalN-induced hepatocyte injury were also measured in vitro. LAE at concentrations of 10–100 μg/ml significantly reduced cellular leakage of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and improved cell viability. The isochlorogenic acids (4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 3,4-O-dicaffeoylquinic acid) at concentrations of 1–100 μg/ml also remarkably improved viability of hepatocytes. The oral treatment of LAE at doses of 50, 100 and 200 mg/kg markedly reduced the serum AST and ALT activity of mice and resulted in significant recovery of hepatocytes in liver sections.
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WU, Y.-H.; HAO, B.-J.; SHEN, E.; MENG, Q.-L.; HU, M.-H.; ZHAO, Y. Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes. Sci. Pharm. 2012, 80, 447-456.
WU Y-H, HAO B-J, SHEN E, MENG Q-L, HU M-H, ZHAO Y. Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes. Scientia Pharmaceutica. 2012; 80(2):447-456.Chicago/Turabian Style
WU, Yi-Hang; HAO, Bing-Jie; SHEN, E; MENG, Qing-Li; HU, Ming-Hui; ZHAO, Yu. 2012. "Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes." Sci. Pharm. 80, no. 2: 447-456.