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Sci. Pharm. 2012, 80(2), 447-456; doi:10.3797/scipharm.1108-16

Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes

1
Department of Pharmacy, College of Life Sciences, China Jiliang University, 310018 Hangzhou, China
2
Intensive Care Unit, Zhejiang Provincial People’s Hospital, 310005 Hangzhou, China
3
Department of Traditional Chinese Medicine and Natural Drug Research, Zhejiang University, 310058 Hangzhou, China
*
Author to whom correspondence should be addressed.
Received: 18 August 2011 / Accepted: 8 February 2012 / Published: 8 February 2012
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Abstract

Laggera alata extract (LAE) was quantitatively analyzed, and its principle components isochlorogenic acids were isolated and authenticated. Protective properties of LAE were studied using a D-galactosamine (D-GalN)-induced injury model in neonatal rat hepatocytes and a D-GalN-induced acute liver damage model in mice. Meanwhile, the effect of isochlorogenic acids derived from LAE on D-GalN-induced hepatocyte injury were also measured in vitro. LAE at concentrations of 10–100 μg/ml significantly reduced cellular leakage of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and improved cell viability. The isochlorogenic acids (4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 3,4-O-dicaffeoylquinic acid) at concentrations of 1–100 μg/ml also remarkably improved viability of hepatocytes. The oral treatment of LAE at doses of 50, 100 and 200 mg/kg markedly reduced the serum AST and ALT activity of mice and resulted in significant recovery of hepatocytes in liver sections.
Keywords: Laggera alata extract; Isochlorogenic acid; D-Galactosamine; Hepatoprotective Laggera alata extract; Isochlorogenic acid; D-Galactosamine; Hepatoprotective
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

WU, Y.-H.; HAO, B.-J.; SHEN, E.; MENG, Q.-L.; HU, M.-H.; ZHAO, Y. Protective Properties of Laggera alata Extract and its Principle Components Against D-Galactosamine-Injured Hepatocytes. Sci. Pharm. 2012, 80, 447-456.

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