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Sci. Pharm. 2012, 80(2), 367-378; doi:10.3797/scipharm.1112-01

Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study

1
Hindu college of Pharmacy, Amaravathi Road, Guntur, Andhrapradesh, 522002, India
2
Jawaharlal Nehru Technological University, Anantapur, 515002, India
3
Donbosco college of Pharmacy, Pulladigunta, Guntur, 522201, India
4
Nirmala college of Pharmacy, Madras road, Kadapa, Andhrapradesh, 516002, India
*
Author to whom correspondence should be addressed.
Received: 1 December 2011 / Accepted: 27 February 2012 / Published: 27 February 2012
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Abstract

A simple, sensitive and selective method has been developed for quantification of Almotriptan (AL) in human plasma using Almotriptan-d6 (ALD6) as an internal standard. Almotriptan and Almotriptan-d6 were detected with proton adducts at m/z 336.1→201.1 and 342.2→207.2 in multiple reaction monitoring (MRM) positive mode, respectively. The method was linear over a concentration range of 0.5–150.0 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) for Almotriptan were 0.2 pg/mL and 0.5 ng/mL, respectively. Liquid-liquid extraction was used followed by MS/MS (ion spray). The method was shown to be precise with an average within-run and between-run variation of 0.68 to 2.78% and 0.57 to 0.86%, respectively. The average within-run and between-run accuracy of the method throughout its linear range was 98.94 to 102.64% and 99.43 to 101.44%, respectively. The mean recovery of drug and internal standard from human plasma was 92.12 ± 4.32% and 89.62 ± 6.32%. It can be applied for clinical and pharmacokinetic studies.
Keywords: HPLC; MS/MS; Almotriptan; Human plasma; Pharmacokinetic study; LLE HPLC; MS/MS; Almotriptan; Human plasma; Pharmacokinetic study; LLE
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

RAVIKUMAR, K.; CHANDU, B.R.; CHALLA, B.R.; CHANDRASEKHAR, K.B. Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study. Sci. Pharm. 2012, 80, 367-378.

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