Synthesis of New 6-{[ω-(Dialkylamino(heterocyclyl)alkyl]thio}- 3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones and Evaluation of their Anticancer and Antimicrobial Activities

Several novel 6-thio-3-R-2-oxo-2H-[1,2,4]triazino[2,3-c]quinazoline-based com-pounds containing an ω-(dialkylamino(heterocyclyl)]alkyl fragment were synthe-sized to examine their anticancer activity. Some of the 6-{[ω-(hetero-cyclyl)alkyl]thio}-3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones (3.1–3.10) were obtained by the nucleophilic substitution of 6-[ω-halogenalkyl]thio-3-R-2H-[1,2,4]triazino[2,3-c]quinazoline-2-ones (2.1–2.8) with azaheterocycles. Alter-natively, compounds 3.1–3.22 were synthesized by alkylation of 3-R-6-thio-2Н-[1,2,4]triazino[2,3-c]quinazoline-2-ones potassium salts (1.1–1.4) with (2-chloro-ethyl)-N,N-dialkylamine hydrochlorides or 1-(2-chloroethyl)heterocycle hydro-chlorides. The structures of compounds were elucidated by ¹H, ¹³C NMR, LC–MS and EI-MS analysis. Then anticancer and antibacterial, biolumi-nescence inhibition of Photobacterium leiognathi Sh1 activities of the sub-stances were tested in vitro. It was found that compound 3.18 possessed a wide range of anticancer activity against 27 cell lines of cancer: non-small cell lung, сolon, CNS, ovarian, renal, prostate, breast, melanoma and leukemia (log GI₅₀ < −5.65). The “structure-activity” relationship was discussed. COMPARE analysis for synthesized anticancer active compounds was performed.