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Sci. Pharm. 2011, 79(3), 555-568; doi:10.3797/scipharm.1101-19

Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride

1
Analytical Development Laboratory, Piramal Healthcare Ltd, Ennore, Chennai 600 057, India
2
Department of Chemistry, Annamalai University, Annamalainagar 608 002, India
3
Research and Development Laboratory, Piramal Healthcare Ltd, Ennore, Chennai 600 057, India
*
Author to whom correspondence should be addressed.
Received: 26 January 2011 / Accepted: 8 May 2011 / Published: 8 May 2011
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Abstract

Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived from two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and mass spectrometry (MS), impurity-1 and impurity-2 were characterized as 2-(3,4-dimethoxyphenyl)-3-methylbut-2-enenitrile and 2-(3,4-dimethoxyphenyl)-2-isopropyl-3-methylbutanenitrile, respectively.
Keywords: Verapamil hydrochloride; NMR; HPLC; MS; Impurity Analysis Verapamil hydrochloride; NMR; HPLC; MS; Impurity Analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

SRINIVASAN, V.; SIVARAMAKRISHNAN, H.; KARTHIKEYAN, B. Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride. Sci. Pharm. 2011, 79, 555-568.

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