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Sci. Pharm. 2009, 77(Short Lectures (SL)), 189; doi:10.3797/scipharm.oephg.21.SL-22 (registering DOI)
Influence of Microplate Mixing on Binding Assays
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstr. 14, 1090, Vienna, Austria
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Abstract Introduction: Microplate-based analytical assays represent one of the most commonly used tools for the sensitive and reproducible detection of bioactive molecules. However, despite the widespread use of microplate assays, the degree of optimisation of the experimental procedures is questionable . Effective mixing is expected to shorten incubation periods, increase reproducibility and sensitivity. To investigate this, the association of cytoadhesive lectins and lectin-modified nanoparticles to epithelial cell monolayers was determined under stationary and mixing conditions.
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FILLAFER, C.; NOWOTNY, R.; WIRTH, M.; GABOR, F. Influence of Microplate Mixing on Binding Assays. Sci. Pharm. 2009, 77, 189.
FILLAFER C, NOWOTNY R, WIRTH M, GABOR F. Influence of Microplate Mixing on Binding Assays. Scientia Pharmaceutica. 2009; 77(Short Lectures (SL)):189.
FILLAFER, C.; NOWOTNY, R.; WIRTH, M.; GABOR, F. 2009. "Influence of Microplate Mixing on Binding Assays." Sci. Pharm. 77, Short Lectures (SL): 189.
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