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Sci. Pharm. 2009, 77(Short Lectures (SL)), 189; doi:10.3797/scipharm.oephg.21.SL-22 (registering DOI)

Influence of Microplate Mixing on Binding Assays

Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstr. 14, 1090, Vienna, Austria
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Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
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Abstract

Introduction: Microplate-based analytical assays represent one of the most commonly used tools for the sensitive and reproducible detection of bioactive molecules. However, despite the widespread use of microplate assays, the degree of optimisation of the experimental procedures is questionable [1]. Effective mixing is expected to shorten incubation periods, increase reproducibility and sensitivity. To investigate this, the association of cytoadhesive lectins and lectin-modified nanoparticles to epithelial cell monolayers was determined under stationary and mixing conditions.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

FILLAFER, C.; NOWOTNY, R.; WIRTH, M.; GABOR, F. Influence of Microplate Mixing on Binding Assays. Sci. Pharm. 2009, 77, 189.

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