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Sci. Pharm. 2009, 77(Short Lectures (SL)), 170; doi:10.3797/scipharm.oephg.21.SL-03 (registering DOI)

How to Deal with Ion Suppression – the Foe of HPLC-MS/MS

Division of Mass Spectrometry and Chromatography, Institute of Medical and Chemical Laboratory Diagnostics (ZIMCL), University Hospital Innsbruck, Austria
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Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
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Abstract

It is well accepted, that a HPLC-MS/MS assay can outperform immunoassays in therapeutic drug monitoring (TDM) due to its increased accuracy, sensitivity, and precision. However, the occurrence of sudden and unpredictable ion yield attenuation, can hamper assay performance. Such “ion suppression effects” are considered a central analytical problem if using an MS/MS detector in bio-analysis. Since pathological patient specimen compositions or individual co-medications may be major causes for ion yield fluctuations, measures have to be taken to evaluate these interferences prior to transferring an HPLC-MS/MS assay to routine use.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

SEGER, C.; GRIESMACHER, A. How to Deal with Ion Suppression – the Foe of HPLC-MS/MS. Sci. Pharm. 2009, 77, 170.

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