Preparation, Quality Control and Biodistribution Studies of two [111In]-Rituximab Immunoconjugates
AbstractIn order to use Auger-electron therapeutic effects in CD20 antigen targeting in lymphomas, Mabthera™ (rituximab) was successively labeled with [111In]-indium chloride (185 MBq) after conjugation with freshly prepared macrocyclic bifunctional chelating agent, N-succinimidyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA-NHS) and ccDTPA separately. Conjugated-Rituximab was obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7.8) to a glass tube pre-coated with freshly prepared DOTA-NHS or ccDTPA (0.01-0.1 mg) at 25°C. Radiolabeling was performed at 37°C in 3h and room temperature for one hour for DOTA-conjugate and DTPA-conjugate respectively. HPLC showed an overall radiochemical purity of 97.5 and 95% for DOTA and DTPA-conjugates respectively (Specific activity =2800-5600 GBq/mM). The final isotonic 111In-rituximab complexes were checked by gel electrophoresis for radiolysis. Preliminary biodistribution studies in normal rat model performed to determine radioimmunoconjugates distribution of up to 48h.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
JALILIAN, A.R.; SARDARI, D.; KIA, L.; ROWSHANFARZAD, P.; GAROUSI, J.; AKHLAGHI, M.; SHANEHSAZZADEH, S.; MIRZAII, M. Preparation, Quality Control and Biodistribution Studies of two [111In]-Rituximab Immunoconjugates. Sci. Pharm. 2008, 76, 151-170.
JALILIAN AR, SARDARI D, KIA L, ROWSHANFARZAD P, GAROUSI J, AKHLAGHI M, SHANEHSAZZADEH S, MIRZAII M. Preparation, Quality Control and Biodistribution Studies of two [111In]-Rituximab Immunoconjugates. Scientia Pharmaceutica. 2008; 76(2):151-170.Chicago/Turabian Style
JALILIAN, Amir R.; SARDARI, Darush; KIA, Leila; ROWSHANFARZAD, Pejman; GAROUSI, Javad; AKHLAGHI, Mehdi; SHANEHSAZZADEH, Saeed; MIRZAII, Mohammad. 2008. "Preparation, Quality Control and Biodistribution Studies of two [111In]-Rituximab Immunoconjugates." Sci. Pharm. 76, no. 2: 151-170.