Next Article in Journal
Synthesis and Preclinical Studies of [61Cu]-N-(2-hydroxyacetophenone)glycinate as a Possible PET Radiopharmaceutical
Previous Article in Journal
Anticonvulsant and Sedative-Hypnotic Activities of N-Acetyl / Methyl Isatin Derivatives
Article Menu

Article Versions

Export Article

Open AccessArticle
Sci. Pharm. 2006, 74(3), 99-119; doi:10.3797/scipharm.2006.74.99 (registering DOI)

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt
*
Author to whom correspondence should be addressed.
Received: 5 January 2006 / Accepted: 22 March 2006 / Published: 30 September 2006
Download PDF [280 KB, uploaded 28 December 2016]

Abstract

A simple and sensitive kinetic spectrophotometric method was developed for the determination of isoxsuprine in pharmaceutical preparations. The method is based upon a kinetic investigation of the coupling reaction between isoxsuprine HCl and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 for a fixed time of 30 min. The absorbance of the yellow coloured product was measured at 395 nm. The absorbance- concentration plot was rectilinear over the range of 2-20 μg mL-1 (r= 0.9994) with minimum detectability of 0.6 μg mL-1 (1.75 x10-6M). The different experimental parameters affecting the development and stability of the colour were carefully studied and optimized. The determination of isoxsuprine by the fixed-concentration and rate-constant methods is also feasible with the calibration equations obtained, but the fixed time method has been found to be more applicable. The proposed method was further applied to the determination of the drug in formulations. The results obtained were in good agreement with those obtained using a reference method. The reaction pathway was proposed.
Keywords: Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations Kinetic determination; isoxsuprine HCl; (NBD-Cl); pharmaceutical preparations
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

El-Enany, N.; Belal, F.; Rizk, M. Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Sci. Pharm. 2006, 74, 99-119.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top