Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Cell Culture
2.3. Measurement of ROS Production
2.4. Cell Treatments, Lysis, Immunoprecipitation, Fractionation and SDS-PAGE
2.5. P47-Phox Kinase Assays
2.6. Fractionation of B-Lymphoblast Cytosol
2.7. Saponin Permeabilization and Inhibition of PKCε
2.8. Data Analysis
3. Results and Discussion
3.1. ROS Production is Increased in PMA-Stimulated Pre-Eclamptic B-LCLs
3.2. Increased Kinase Activity and P47-Phox Phosphorylation in B-LCLs
3.3. P47-Phox Phosphorylation Is Increased in Pre-Eclamptic B-LCLs
3.4. PKC Expression Is Unchanged between Normal and Pre-Eclamptic B-LCLs
3.5. P47-Phox Kinase Activity Is Increased in Pre-Eclamptic B-LCLs
3.6. Analysis of P47-Phox Kinase Activity in Pre-Eclamptic B-LCLs
3.7. PKCε Activation in Pre-Eclamptic B-LCLs
4. Conclusions
Supplementary Materials
Acknowledgments
Conflicts of Interest
References
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Poolman, T.M.; Quinn, P.A.; Ng, L. Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model. Diseases 2013, 1, 1-17. https://doi.org/10.3390/diseases1010001
Poolman TM, Quinn PA, Ng L. Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model. Diseases. 2013; 1(1):1-17. https://doi.org/10.3390/diseases1010001
Chicago/Turabian StylePoolman, Toryn M., Paulene A. Quinn, and Leong Ng. 2013. "Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model" Diseases 1, no. 1: 1-17. https://doi.org/10.3390/diseases1010001