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Cosmetics 2017, 4(3), 23; doi:10.3390/cosmetics4030023

Evaluation of Genotoxic and DNA Photo-Protective Activity of Bryothamnion triquetrum and Halimeda incrassata Seaweeds Extracts

1
Plant Biology Department, Faculty of Biology, University of Havana, Havana 10400, Cuba
2
Radiobiology Department, Center of Applied Technology and Nuclear Development (CEADEN), Havana 11300, Cuba
3
Microbiology Department, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 03178-200, Brazil
*
Authors to whom correspondence should be addressed.
Received: 17 May 2017 / Revised: 2 July 2017 / Accepted: 7 July 2017 / Published: 13 July 2017
(This article belongs to the Special Issue Cosmetics from Marine Sources)
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Abstract

The ultraviolet (UV) component of sunlight is high on the earth surface, especially at low latitudes, raising the risk of skin diseases, including cancer. The use of natural compounds is a strategy to protect people against UV damage. Seaweeds are becoming increasingly influential in the food industry, and are also used in the pharmacy and cosmetic industries, due to several bioactive demonstrated properties. This work analyzed the genotoxic and photoprotective effects of the aqueous extracts of two seaweed species: Bryothamnion triquetrum and Halimeda incrassata. A cell-free plasmid DNA assay was employed, allowing detection of DNA breaks. The plasmids were exposed to increasing concentrations of aqueous extracts. DNA break was produced at concentrations of 2.0 and 4.0 mg/mL in both seaweed extracts and, consequently, a genotoxic effect is postulated. This effect arises with higher exposure times. Additionally, different combinations of plasmid DNA, restriction enzymes (Eco RI, Bam HI, and Pvu II) and extracts were assayed. The extracts did not produce an interference effect in the reconnaissance of the specific restriction target sequences of each enzyme. Photoprotective activity of the extracts was evaluated in UVC-irradiated plasmids. None of the extracts displayed DNA protective effects in this assay. View Full-Text
Keywords: plasmid DNA; UV radiation; DNA protection plasmid DNA; UV radiation; DNA protection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sánchez-Lamar, Á.; González-Pumariega, M.; Fuentes-León, F.; Vernhes Tamayo, M.; Schuch, A.P.; Menck, C.F.M. Evaluation of Genotoxic and DNA Photo-Protective Activity of Bryothamnion triquetrum and Halimeda incrassata Seaweeds Extracts. Cosmetics 2017, 4, 23.

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