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Biology 2015, 4(2), 251-263; doi:10.3390/biology4020251

Modulation of Group I Ribozyme Activity by Cationic Porphyrins

1
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan
2
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Moto-oka 744, Nishi-ku, Fukuoka 819-0395, Japan
3
Center for Molecular Systems, Kyushu University, Moto-oka 744, Nishi-ku, Fukuoka 819-0395, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Chris O'Callaghan
Received: 1 December 2014 / Accepted: 3 March 2015 / Published: 24 March 2015
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Abstract

The effects of cationic porphyrins on the catalytic activities of four group I ribozymes were investigated. A cationic porphyrin possessing four pyridinium moieties (pPyP) inhibited two group IC3 ribozymes (Syn Rz and Azo Rz) and a group IC1 ribozyme (Tet Rz). In the case of a group IA2 ribozyme (Td Rz), however, pPyP served not only as an inhibitor but also as an activator, and the effects of pPyP were dependent on its concentration. To analyze the structural and electronic factors determining the effects of pPyP on group I ribozymes, three cationic porphyrins (pPyNCP, pPyF4P, and TMPyP) were also examined. As interactions between small organic molecules and nucleic acids are attractive and important issues in biochemistry and biotechnology, this study contributes to the development of porphyrin-based molecules that can modulate functions of structured RNA molecules. View Full-Text
Keywords: RNA; ribozyme; porphyrin RNA; ribozyme; porphyrin
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MDPI and ACS Style

Matsumura, S.; Ito, T.; Tanaka, T.; Furuta, H.; Ikawa, Y. Modulation of Group I Ribozyme Activity by Cationic Porphyrins. Biology 2015, 4, 251-263.

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