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Biology 2015, 4(1), 67-87; doi:10.3390/biology4010067

In Vivo Molecular Responses of Fast and Slow Muscle Fibers to Lipopolysaccharide in a Teleost Fish, the Rainbow Trout (Oncorhynchus mykiss)

1
Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona and Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona 08028, Spain
2
Institut de Biotecnologia i de Biomedicina-Parc de Recerca UAB, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain
3
Nofima, Osloveien 1, N-1432 Ås, Norway
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Jim O. Vigoreaux
Received: 27 November 2014 / Revised: 6 January 2015 / Accepted: 28 January 2015 / Published: 4 February 2015
(This article belongs to the Special Issue Muscle Structure and Function)
View Full-Text   |   Download PDF [676 KB, uploaded 4 February 2015]   |  

Abstract

The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout. View Full-Text
Keywords: muscle; lipopolysaccharide; transcriptome; proteome; fish muscle; lipopolysaccharide; transcriptome; proteome; fish
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Magnoni, L.J.; Roher, N.; Crespo, D.; Krasnov, A.; Planas, J.V. In Vivo Molecular Responses of Fast and Slow Muscle Fibers to Lipopolysaccharide in a Teleost Fish, the Rainbow Trout (Oncorhynchus mykiss). Biology 2015, 4, 67-87.

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