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Biology 2015, 4(1), 237-250; doi:10.3390/biology4010237

Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge

1
National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington LE12 5RD, UK
2
Department of Food Engineering, Abant Izzet Baysal University, Bolu 14280, Turkey
3
Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2RD, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Thorsten Berg
Received: 1 December 2014 / Revised: 8 February 2015 / Accepted: 16 February 2015 / Published: 6 March 2015
(This article belongs to the Special Issue Protein-Protein Interactions)
View Full-Text   |   Download PDF [668 KB, uploaded 6 March 2015]   |  

Abstract

Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions. View Full-Text
Keywords: protein; carbohydrate; nucleic acid; interaction; hydrodynamics protein; carbohydrate; nucleic acid; interaction; hydrodynamics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Harding, S.E.; Gillis, R.B.; Almutairi, F.; Erten, T.; Kök, M.Ş.; Adams, G.G. Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge. Biology 2015, 4, 237-250.

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