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A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses
School of Optometry and Vision Science, The University of New South Wales, Sydney NSW 2052, Australia
Brien Holden Vision Institute, Sydney NSW 2052, Australia
* Author to whom correspondence should be addressed.
Received: 18 July 2013; in revised form: 1 October 2013 / Accepted: 29 October 2013 / Published: 1 November 2013
Abstract: Adhesion of pathogenic microbes, particularly bacteria, to contact lenses is implicated in contact lens related microbial adverse events. Various in vitro conditions such as type of bacteria, the size of initial inoculum, contact lens material, nutritional content of media, and incubation period can influence bacterial adhesion to contact lenses and the current study investigated the effect of these conditions on bacterial adhesion to contact lenses. There was no significant difference in numbers of bacteria that adhered to hydrogel etafilcon A or silicone hydrogel senofilcon A contact lenses. Pseudomonas aeruginosa adhered in higher numbers compared to Staphylococcus aureus. Within a genera/species, adhesion of different bacterial strains did not differ appreciably. The size of initial inoculum, nutritional content of media, and incubation period played significant roles in bacterial adhesion to lenses. A set of in vitro assay conditions to help standardize adhesion between studies have been recommended.
Keywords: Bacterial adhesion; contact lens; Pseudomonas aeruginosa; Staphylococcus aureus
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Cite This Article
MDPI and ACS Style
Dutta, D.; Willcox, M.D. A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses. Biology 2013, 2, 1268-1281.
Dutta D, Willcox MD. A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses. Biology. 2013; 2(4):1268-1281.
Dutta, Debarun; Willcox, Mark D. 2013. "A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses." Biology 2, no. 4: 1268-1281.