Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Biology 2013, 2(1), 142-150; doi:10.3390/biology2010142
Article

Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes

1
, 2
, 2
, 2,*  and 1,3,*
Received: 6 December 2012; in revised form: 27 December 2012 / Accepted: 31 December 2012 / Published: 14 January 2013
(This article belongs to the Special Issue Polar Microbiology: Recent Advances and Future Perspectives)
View Full-Text   |   Download PDF [514 KB, uploaded 14 January 2013]
Abstract: Three psychrophilic protein pheromones (En-1, En-2 and En-6) from the polar ciliate, Euplotes nobilii, and six mesophilic pheromones (Er-1, Er-2, Er-10, Er-11, Er-22 and Er-23) from the temperate-water sister species, Euplotes raikovi, were studied in aqueous solution for their thermal unfolding and refolding based on the temperature dependence of their circular dichroism (CD) spectra. The three psychrophilic proteins showed thermal unfolding with mid points in the temperature range 55–70 °C. In contrast, no unfolding was observed for any of the six mesophilic proteins and their regular secondary structures were maintained up to 95 °C. Possible causes of these differences are discussed based on comparisons of the NMR structures of the nine proteins.
Keywords: protein denaturation; protein stability; circular dichroism spectroscopy; psychrophilic proteins; chemical signals protein denaturation; protein stability; circular dichroism spectroscopy; psychrophilic proteins; chemical signals
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Geralt, M.; Alimenti, C.; Vallesi, A.; Luporini, P.; Wüthrich, K. Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes. Biology 2013, 2, 142-150.

AMA Style

Geralt M, Alimenti C, Vallesi A, Luporini P, Wüthrich K. Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes. Biology. 2013; 2(1):142-150.

Chicago/Turabian Style

Geralt, Michael; Alimenti, Claudio; Vallesi, Adriana; Luporini, Pierangelo; Wüthrich, Kurt. 2013. "Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes." Biology 2, no. 1: 142-150.



Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert