Next Article in Journal
In Situ Hybridization of Pulp Fibers Using Mg-Al Layered Double Hydroxides
Previous Article in Journal
Novel Hybrid Flax Reinforced Supersap Composites in Automotive Applications
Article Menu

Export Article

Open AccessArticle
Fibers 2015, 3(2), 90-102; doi:10.3390/fib3020090

Design and Construction of Large Amyloid Fibers

Biological Systems Engineering Department, Virginia Tech, 301D HABB1, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen C. Bondy
Received: 3 January 2015 / Accepted: 1 April 2015 / Published: 16 April 2015
View Full-Text   |   Download PDF [631 KB, uploaded 16 April 2015]   |  

Abstract

Mixtures of “template” and “adder” proteins self-assemble into large amyloid fibers of varying morphology and modulus. Fibers range from low modulus, rectangular cross-sectioned tapes to high modulus, circular cross-sectioned cylinders. Varying the proteins in the mixture can elicit “in-between” morphologies, such as elliptical cross-sectioned fibers and twisted tapes, both of which have moduli in-between rectangular tapes and cylindrical fibers. Experiments on mixtures of proteins of known amino acid sequence show that control of the large amyloid fiber morphology is dependent on the amount of glutamine repeats or “Q-blocks” relative to hydrophobic side chained amino acids such as alanine, isoleucine, leucine, and valine in the adder protein. Adder proteins with only hydrophobic groups form low modulus rectangular cross-sections and increasing the Q-block content allows excess hydrogen bonding on amide groups that results in twist and higher modulus. The experimental results show that large amyloid fibers of specific shape and modulus can be designed and controlled at the molecular level. View Full-Text
Keywords: amyloid; fiber; β-sheet; protein amyloid; fiber; β-sheet; protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ridgley, D.M.; Rippner, C.M.W.; Barone, J.R. Design and Construction of Large Amyloid Fibers. Fibers 2015, 3, 90-102.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Fibers EISSN 2079-6439 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top