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Antibiotics 2015, 4(4), 455-466; doi:10.3390/antibiotics4040455

Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing

1
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
2
Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA
Current address: Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 1700 4th Street, San Francisco, CA 94158, USA.
Current address: Analytical Development Laboratories, CMC Center, Takeda Pharmaceutical Company Ltd., 17-85 Jusohonmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan.
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher C. Butler
Received: 15 June 2015 / Revised: 4 October 2015 / Accepted: 22 October 2015 / Published: 29 October 2015
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Abstract

Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm) that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922) treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method. View Full-Text
Keywords: antibiotics; AST; microfluidics; microdevice; lab-on-a-chip; MIC antibiotics; AST; microfluidics; microdevice; lab-on-a-chip; MIC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kim, S.C.; Cestellos-Blanco, S.; Inoue, K.; Zare, R.N. Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing. Antibiotics 2015, 4, 455-466.

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