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Biosensors 2018, 8(3), 81; https://doi.org/10.3390/bios8030081

Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

1
Oritest spol. s r.o., Nábřežní 90/4, 150 00 Prague, Czech Republic
2
Faculty of Environmental Technology, University of Chemistry and Technology, Technická 5, 166 28 Prague, Czech Republic
3
National Institute for Nuclear, Chemical and Biological Protection, Kamenná 71, 262 31 Milín, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 10 July 2018 / Revised: 27 August 2018 / Accepted: 29 August 2018 / Published: 4 September 2018
(This article belongs to the Special Issue Biosensors for Environmental Applications)
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Abstract

Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman’s reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%). View Full-Text
Keywords: biosensor of cholinesterase inhibitors; chemical warfare agents; enzymatic reaction; Guinea Green B; visual evaluation biosensor of cholinesterase inhibitors; chemical warfare agents; enzymatic reaction; Guinea Green B; visual evaluation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Pitschmann, V.; Matějovský, L.; Lobotka, M.; Dědič, J.; Urban, M.; Dymák, M. Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator. Biosensors 2018, 8, 81.

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