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Biosensors 2018, 8(3), 70; https://doi.org/10.3390/bios8030070

3D Carbon Microelectrodes with Bio-Functionalized Graphene for Electrochemical Biosensing

1
Department of Micro- and Nanotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
2
Department of Chemistry, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
*
Author to whom correspondence should be addressed.
Received: 15 May 2018 / Revised: 2 July 2018 / Accepted: 5 July 2018 / Published: 19 July 2018
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Abstract

An enzyme-based electrochemical biosensor has been developed with 3D pyrolytic carbon microelectrodes that have been coated with bio-functionalized reduced graphene oxide (RGO). The 3D carbon working electrode was microfabricated using the pyrolysis of photoresist precursor structures, which were subsequently functionalized with graphene oxide and enzymes. Glucose detection was used to compare the sensor performance achieved with the 3D carbon microelectrodes (3DCMEs) to the 2D electrode configuration. The 3DCMEs provided an approximately two-fold higher sensitivity of 23.56 µA·mM−1·cm−2 compared to 10.19 µA mM−1·cm−2 for 2D carbon in glucose detection using cyclic voltammetry (CV). In amperometric measurements, the sensitivity was more than 4 times higher with 0.39 µA·mM−1·cm−2 for 3D electrodes and 0.09 µA·mM−1·cm−2 for the 2D configuration. The stability analysis of the enzymes on the 3D carbon showed reproducible results over 7 days. The selectivity of the electrode was evaluated with solutions of glucose, uric acid, cholesterol and ascorbic acid, which showed a significantly higher response for glucose. View Full-Text
Keywords: 3D carbon microelectrodes; graphene oxide; glucose; electrochemical biosensor 3D carbon microelectrodes; graphene oxide; glucose; electrochemical biosensor
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Hemanth, S.; Halder, A.; Caviglia, C.; Chi, Q.; Keller, S.S. 3D Carbon Microelectrodes with Bio-Functionalized Graphene for Electrochemical Biosensing. Biosensors 2018, 8, 70.

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