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Biosensors 2017, 7(3), 34; doi:10.3390/bios7030034

Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques

1
Departamento de Química, Facultad de Ciencias Naturales, Matemáticas y del Medio Ambiente, Universidad Tecnológica Metropolitana, P.O. Box 9845, Correo 21, Santiago 7800003, Chile
2
Departamento de Ingeniería Estructural y Geotecnia, Escuela de Ingeniería, Pontificia Universidad Católica de Chile, P.O. Box 306, Correo 22, Santiago 7820436, Chile
3
Facultad de Química, Pontificia Universidad Católica de Chile, P.O. Box 306, Correo 22, Santiago 7820436, Chile
*
Author to whom correspondence should be addressed.
Received: 13 July 2017 / Revised: 15 August 2017 / Accepted: 16 August 2017 / Published: 18 August 2017
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Abstract

Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affected by the aliphatic chain length, saturations, and molecule polarity, among others. Bilayer stability becomes a fundamental factor when technological devices are developed—like biosensors—based on those systems. Thermal studies were performed for different types of phosphatidylcholine (PC) molecules: two pure PC bilayers and four binary PC mixtures. These analyses were carried out through the detection of slight changes in their optical and structural parameters via Ellipsometry and Surface Plasmon Resonance (SPR) techniques. Phospholipid bilayers were prepared by Langmuir-Blodgett technique and deposited over a hydrophilic silicon wafer. Their molecular inclination degree, mobility, and stability of the different phases were detected and analyzed through bilayer thickness changes and their optical phase-amplitude response. Results show that certain binary lipid mixtures—with differences in its aliphatic chain length—present a co-existence of two thermal responses due to non-ideal mixing. View Full-Text
Keywords: binary phospholipid mixture; heterogeneous membranes; thermal phase transitions; ellipsometry; surface plasmon resonance binary phospholipid mixture; heterogeneous membranes; thermal phase transitions; ellipsometry; surface plasmon resonance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

González-Henríquez, C.M.; Villegas-Opazo, V.A.; Sagredo-Oyarce, D.H.; Sarabia-Vallejos, M.A.; Terraza, C.A. Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques. Biosensors 2017, 7, 34.

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