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Biosensors 2017, 7(3), 33; doi:10.3390/bios7030033

Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment

1
Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy
2
Regina Elena National Cancer Institute, Via E. Chianesi 53, 00144 Rome, Italy
3
Fraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, Germany
4
Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstr. 28, 01277 Dresden, Germany
5
IBI-Istituto Biochimico Italiano Giovanni Lorenzini Spa, Via Fossignano 2, 04011 Aprilia, Italy
Present Address: Bambino Gesù Pediatric Hospital, Viale S. Paolo 15, 00146 Rome, Italy.
*
Author to whom correspondence should be addressed.
Received: 27 June 2017 / Revised: 11 August 2017 / Accepted: 15 August 2017 / Published: 17 August 2017
(This article belongs to the Special Issue Biosensors for the Detection of Biomarkers)
View Full-Text   |   Download PDF [3227 KB, uploaded 17 August 2017]   |  

Abstract

We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up which operates in both label-free and fluorescence modes. The detection approach makes use of a sandwich assay, in which the one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies, in order to guarantee high specificity during the biological recognition. We present the results of exemplary protein G based label-free assays in complex biological matrices, reaching an estimated limit of detection of 0.5 ng/mL. On-chip and chip-to-chip variability of the results is addressed too, providing repeatability rates. Moreover, results on fluorescence operation demonstrate the capability to perform high sensitive cancer biomarker assays reaching a resolution of 0.6 ng/mL, without protein G assistance. The resolution obtained in both modes meets international guidelines and recommendations (15 ng/mL) for ERBB2 quantification assays, providing an alternative tool to phenotype and diagnose molecular cancer subtypes. View Full-Text
Keywords: Optical biosensors; Bloch surface waves; 1D photonic crystals; ERBB2; SK-BR 3; Breast cancer Optical biosensors; Bloch surface waves; 1D photonic crystals; ERBB2; SK-BR 3; Breast cancer
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MDPI and ACS Style

Sinibaldi, A.; Sampaoli, C.; Danz, N.; Munzert, P.; Sonntag, F.; Centola, F.; Occhicone, A.; Tremante, E.; Giacomini, P.; Michelotti, F. Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment. Biosensors 2017, 7, 33.

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