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Biosensors 2017, 7(1), 12; doi:10.3390/bios7010012

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain
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Author to whom correspondence should be addressed.
Academic Editor: Laurent A. Francis
Received: 13 January 2017 / Revised: 17 February 2017 / Accepted: 26 February 2017 / Published: 1 March 2017
(This article belongs to the Special Issue Systems for the Early Detection of Pathogenic Bacteria)
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Abstract

Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture. View Full-Text
Keywords: C-type lectin; agglutination; CRD; bacterial detection; E. coli C-type lectin; agglutination; CRD; bacterial detection; E. coli
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MDPI and ACS Style

Gasmi, L.; Ferré, J.; Herrero, S. High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae). Biosensors 2017, 7, 12.

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