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Biosensors 2016, 6(4), 49; doi:10.3390/bios6040049

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

1
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA
2
Currently at Cepheid, Sunnyvale, CA 94089, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Laurent A. Francis
Received: 25 August 2016 / Revised: 16 September 2016 / Accepted: 20 September 2016 / Published: 23 September 2016
(This article belongs to the Special Issue Systems for the Early Detection of Pathogenic Bacteria)
View Full-Text   |   Download PDF [2074 KB, uploaded 23 September 2016]   |  

Abstract

Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. This platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min. View Full-Text
Keywords: microfluidics; diagnostics; pathogen detection; point-of-care; immunoassay; centrifugal; enteric diseases microfluidics; diagnostics; pathogen detection; point-of-care; immunoassay; centrifugal; enteric diseases
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Phaneuf, C.R.; Mangadu, B.; Piccini, M.E.; Singh, A.K.; Koh, C.-Y. Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices. Biosensors 2016, 6, 49.

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