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Biosensors 2015, 5(2), 141-157; doi:10.3390/bios5020141

Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells

1
Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy
2
Rowland Institute, Harvard University, Cambridge, MA 02142, USA
3
Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrew M. Shaw
Received: 16 January 2015 / Revised: 18 February 2015 / Accepted: 24 March 2015 / Published: 1 April 2015
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
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Abstract

A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols. View Full-Text
Keywords: spermatozoa; biophotonics; sex-identification; digital holography microscopy; Raman spectroscopy; Raman imaging spermatozoa; biophotonics; sex-identification; digital holography microscopy; Raman spectroscopy; Raman imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ferrara, M.A.; Di Caprio, G.; Managò, S.; De Angelis, A.; Sirleto, L.; Coppola, G.; De Luca, A.C. Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. Biosensors 2015, 5, 141-157.

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