Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells
AbstractA full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols. View Full-Text
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Ferrara, M.A.; Di Caprio, G.; Managò, S.; De Angelis, A.; Sirleto, L.; Coppola, G.; De Luca, A.C. Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. Biosensors 2015, 5, 141-157.
Ferrara MA, Di Caprio G, Managò S, De Angelis A, Sirleto L, Coppola G, De Luca AC. Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. Biosensors. 2015; 5(2):141-157.Chicago/Turabian Style
Ferrara, Maria A.; Di Caprio, Giuseppe; Managò, Stefano; De Angelis, Annalisa; Sirleto, Luigi; Coppola, Giuseppe; De Luca, Anna C. 2015. "Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells." Biosensors 5, no. 2: 141-157.