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Biosensors 2015, 5(1), 98-117; doi:10.3390/bios5010098

Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance

1
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan
2
Department of Engineering and System Science, National Tsing Hua University, Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan
3
Institute of Biomedical Science, Academia Sinica, Taipei 11529, Taiwan
4
Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan
5
Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Keelung 202, Taiwan
6
Ph.D. Program in Microbial Genomics, National Chung Hsing University, Taichung 402, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew M. Shaw
Received: 30 December 2014 / Revised: 7 March 2015 / Accepted: 10 March 2015 / Published: 23 March 2015
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
View Full-Text   |   Download PDF [1096 KB, uploaded 23 March 2015]   |  

Abstract

Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits. View Full-Text
Keywords: Surface Plasmon Resonance; gold nanoslits; rare cells; double capture; magnetic nanoparticles; microfluidic chip Surface Plasmon Resonance; gold nanoslits; rare cells; double capture; magnetic nanoparticles; microfluidic chip
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MDPI and ACS Style

Mousavi, M.Z.; Chen, H.-Y.; Hou, H.-S.; Chang, C.-Y.-Y.; Roffler, S.; Wei, P.-K.; Cheng, J.-Y. Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance. Biosensors 2015, 5, 98-117.

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