Label-Free Sensing of Adenosine Based on Force Variations Induced by Molecular Recognition
AbstractWe demonstrate a simple force-based label-free strategy for the highly sensitive sensing of adenosine. An adenosine ssDNA aptamer was bound onto an atomic force microscopy (AFM) probe by covalent modification, and the molecular-interface adsorption force between the aptamer and a flat graphite surface was measured by single-molecule force spectroscopy (SMFS). In the presence of adenosine, the molecular recognition between adenosine and the aptamer resulted in the formation of a folded, hairpin-like DNA structure and hence caused a variation of the adsorption force at the graphite/water interface. The sensitive force response to molecular recognition provided an adenosine detection limit in the range of 0.1 to 1 nM. The addition of guanosine, cytidine, and uridine had no significant interference with the sensing of adenosine, indicating a strong selectivity of this sensor architecture. In addition, operational parameters that may affect the sensor, such as loading rate and solution ionic strength, were investigated. View Full-Text
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Li, J.; Li, Q.; Ciacchi, L.C.; Wei, G. Label-Free Sensing of Adenosine Based on Force Variations Induced by Molecular Recognition. Biosensors 2015, 5, 85-97.
Li J, Li Q, Ciacchi LC, Wei G. Label-Free Sensing of Adenosine Based on Force Variations Induced by Molecular Recognition. Biosensors. 2015; 5(1):85-97.Chicago/Turabian Style
Li, Jingfeng; Li, Qing; Ciacchi, Lucio C.; Wei, Gang. 2015. "Label-Free Sensing of Adenosine Based on Force Variations Induced by Molecular Recognition." Biosensors 5, no. 1: 85-97.