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Biosensors 2014, 4(4), 358-369; doi:10.3390/bios4040358

Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches

1
Department of Physical Chemistry, Chemistry Institute, São Paulo State University (UNESP), 55 Prof. Francisco Degni Street, 14800-060, São Paulo, Brazil
2
Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (USP), 3900 Bandeirantes Avenue, 14049-900, Ribeirão Preto, São Paulo, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 June 2014 / Revised: 25 August 2014 / Accepted: 15 September 2014 / Published: 3 October 2014
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
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Abstract

Label-free methods for evaluating lectin–cell binding have been developed to determine the lectin–carbohydrate interactions in the context of cell-surface oligosaccharides. In the present study, mass loading and electrochemical transducer signals were compared to characterize the interaction between lectin and cellular membranes by measuring the equilibrium association constant, , between ArtinM lectin and the carbohydrate sites of NB4 leukemia cells. By functionalizing sensor interfaces with ArtinM, it was possible to determine over a range of leukemia cell concentrations to construct analytical curves from impedimetric and/or mass-associated frequency shifts with analytical signals following a Langmuir pattern. Using the Langmuir isotherm-binding model, the obtained were (8.9 ± 1.0) × 10−5 mL/cell and (1.05 ± 0.09) × 10−6 mL/cell with the electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) methods, respectively. The observed differences were attributed to the intrinsic characteristic sensitivity of each method in following Langmuir isotherm premises. View Full-Text
Keywords: ArtinM; lectin; myeloid leukemia cells; electrochemical impedance spectroscopy; quartz crystal microbalance; Langmuir isotherm; equilibrium association constant (Ka) ArtinM; lectin; myeloid leukemia cells; electrochemical impedance spectroscopy; quartz crystal microbalance; Langmuir isotherm; equilibrium association constant (Ka)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Carvalho, F.C.; Martins, D.C.; Santos, A.; Roque-Barreira, M.-C.; Bueno, P.R. Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches. Biosensors 2014, 4, 358-369.

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