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Biosensors 2014, 4(3), 257-272; doi:10.3390/bios4030257

Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

1
Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark
2
DELTA Dansk Elektronik, Lys & Akustik, Venlighedsvej 4, DK-2970 Hørsholm, Denmark
These authors contributed equally to this work.
Present address: Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, 08193 Bellaterra, Spain.
*
Author to whom correspondence should be addressed.
Received: 20 May 2014 / Revised: 5 August 2014 / Accepted: 8 August 2014 / Published: 13 August 2014
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
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Abstract

This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy. View Full-Text
Keywords: electrical impedance spectroscopy; HeLa cells; paclitaxel; cell properties electrical impedance spectroscopy; HeLa cells; paclitaxel; cell properties
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kirkegaard, J.; Clausen, C.H.; Rodriguez-Trujillo, R.; Svendsen, W.E. Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry. Biosensors 2014, 4, 257-272.

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