Biosensors 2014, 4(2), 137-149; doi:10.3390/bios4020137
A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
Louise Elmlund 1,2,†, Pernilla Söderberg 1,2,†, Subramanian Suriyanarayanan 1,2 and Ian A. Nicholls 1,2,3,*
1
Bioorganic & Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry, Linnæus University, SE-39182 Kalmar, Sweden
2
Department of Chemistry & Biomedical Sciences, Linnæus University, SE-39182 Kalmar, Sweden
3
Department of Chemistry—BMC, Uppsala University, Box 576, SE-75123 Uppsala, Sweden
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 March 2014 / Revised: 11 April 2014 / Accepted: 23 April 2014 / Published: 29 April 2014
(This article belongs to the Special Issue Piezoelectric Biosensors)
Abstract
Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (KD of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions. View Full-TextKeywords:
phage display; piezoelectric sensor; QCM; adenine; peptide; molecular recognition
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Elmlund, L.; Söderberg, P.; Suriyanarayanan, S.; Nicholls, I.A. A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety. Biosensors 2014, 4, 137-149.
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