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Biosensors 2012, 2(2), 127-170; doi:10.3390/bios2020127

Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China
Author to whom correspondence should be addressed.
Received: 2 March 2012 / Revised: 2 April 2012 / Accepted: 19 April 2012 / Published: 25 April 2012
(This article belongs to the Special Issue Electrochemical Based Biosensors)


Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.
Keywords: electrochemical biosensor; cell-based biosensor; microfabrication; in vitro monitoring electrochemical biosensor; cell-based biosensor; microfabrication; in vitro monitoring

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wang, J.; Wu, C.; Hu, N.; Zhou, J.; Du, L.; Wang, P. Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro. Biosensors 2012, 2, 127-170.

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