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Biosensors 2012, 2(1), 32-42; doi:10.3390/bios2010032
Article

Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

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Received: 8 December 2011 / Revised: 6 January 2012 / Accepted: 16 January 2012 / Published: 18 January 2012
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Abstract

Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, with relevance in human and animal health in sub-Saharan Africa. The intensity of the chemiluminescent signal was evaluated by using a charge-coupled device as well as a microtiter plate reader. We demonstrated that our lateral flow chemiluminescent assay has a very low limit of detection and is easy to use. The limit of detection was determined to be 0.5 fmols of nucleic acid target.
Keywords: biosensor; lateral flow assay; Trypanosoma; chemiluminescence biosensor; lateral flow assay; Trypanosoma; chemiluminescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, Y.; Fill, C.; Nugen, S.R. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids. Biosensors 2012, 2, 32-42.

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