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Biosensors 2012, 2(1), 15-31; doi:10.3390/bios2010015
Article

Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

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Received: 16 December 2011 / Revised: 4 January 2012 / Accepted: 16 January 2012 / Published: 17 January 2012
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Abstract

Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.
Keywords: electrochemical biosensor; pathogen detection; magnetic polyaniline; screen-printed carbon electrode; cyclic voltammetry electrochemical biosensor; pathogen detection; magnetic polyaniline; screen-printed carbon electrode; cyclic voltammetry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Setterington, E.B.; Alocilja, E.C. Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens. Biosensors 2012, 2, 15-31.

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