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Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA
* Author to whom correspondence should be addressed.
Received: 16 December 2011; in revised form: 4 January 2012 / Accepted: 16 January 2012 / Published: 17 January 2012
Abstract: Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.
Keywords: electrochemical biosensor; pathogen detection; magnetic polyaniline; screen-printed carbon electrode; cyclic voltammetry
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MDPI and ACS Style
Setterington, E.B.; Alocilja, E.C. Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens. Biosensors 2012, 2, 15-31.
Setterington EB, Alocilja EC. Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens. Biosensors. 2012; 2(1):15-31.
Setterington, Emma B.; Alocilja, Evangelyn C. 2012. "Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens." Biosensors 2, no. 1: 15-31.