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Nanomaterials 2018, 8(4), 258; https://doi.org/10.3390/nano8040258

Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination

1
,
2
and
1,*
1
Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education and Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture China Agricultural University, Beijing 100083, China
2
School of Electronic and Information Engineering, Xi’an Jiaotong University, Xi’an 710049, China
*
Author to whom correspondence should be addressed.
Received: 19 March 2018 / Revised: 17 April 2018 / Accepted: 18 April 2018 / Published: 20 April 2018
(This article belongs to the Special Issue Applications of Carbon Nanotubes)
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Abstract

Silver, a very common heavy metal, has been employed in electronics, medicine, jewelry, and catalysis due to its excellent chemical and physical characteristics. Silver-containing wastes can cause environmental pollution, so it is vital to monitor the Ag(I) concentration. Here, a label-free biosensor was developed for the Ag(I) detection, which used single-walled carbon nanotubes/field effect transistor (SWNTs/FET) to functionalize with a specific DNAzyme, containing an Agzyme and a complementary strand DNA (CS-DNA) embedded an RNA-base. The CS-DNA was covalently immobilized on the SWNTs’ surface through peptide bonds, and then combined with the Agzyme. When Ag(I) was bound with the Agzyme, the CS-DNA can be cleaved at the RNA site efficiently. The cleaved DNAzyme induced a remarkable change in the electrical conductivity of SWNTs. The performances of DNAzyme/SWNTs/FET were investigated using different spectroscopy and electrochemical methods. Under the optimized parameters, DNAzyme/SWNTs/FET presented a high sensitivity and selectivity towards Ag(I), in which the linear response range is 10 pM to 106 pM and the limit of detection is 5 pM(S/N = 3). Additionally, the prepared biosensor was applied to measure the Ag(I) concentration in the water sample with good results. View Full-Text
Keywords: field effect transistor; single-walled carbon nanotubes; silver ion; biosensor; DNAzyme field effect transistor; single-walled carbon nanotubes; silver ion; biosensor; DNAzyme
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, H.; Liu, Y.; Liu, G. Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination. Nanomaterials 2018, 8, 258.

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