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Nanomaterials 2018, 8(2), 107; https://doi.org/10.3390/nano8020107

Qualitative and Quantitative Detection of PrPSc Based on the Controlled Release Property of Magnetic Microspheres Using Surface Plasmon Resonance (SPR)

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210039, China
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Received: 7 January 2018 / Revised: 8 February 2018 / Accepted: 12 February 2018 / Published: 13 February 2018
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Abstract

Prion protein (PrPSc) has drawn widespread attention due to its pathological potential to prion diseases. In this work, we constructed a novel surface plasmon resonance (SPR) detection assay involving magnetic microspheres (MMs) and its controlled release property, for selective capture, embedding, concentration, and SPR detection of PrPSc with high sensitivity and specificity. Aptamer-modified magnetic particles (AMNPs) were used to specifically capture PrPSc. Amphiphilic copolymer was used to embed the labeled PrPSc and form magnetic microspheres to isolate PrPSc from the external environment. Static magnetic and alternating magnetic fields were used to concentrate and control release the embedded PrPSc, respectively. Finally, the released AMNPs-labeled PrPSc was detected by SPR which was equipped with a bare gold sensing film. A good linear relationship was obtained between SPR responses and the logarithm of PrPSc concentrations over a range of 0.01–1000 ng/mL. The detection sensitivity for PrPSc was improved by 10 fold compared with SPR direct detection format. The specificity of the present biosensor was also determined by PrPC and other reagents as controls. This proposed approach could also be used to isolate and detect other highly pathogenic biomolecules with similar structural characteristics by altering the corresponding aptamer in the AMNPs conjugates. View Full-Text
Keywords: surface plasmon resonance 1; prion protein 2; aptamer 3; magnetic microsphere 4; self-assembling 5; sensitive 6 surface plasmon resonance 1; prion protein 2; aptamer 3; magnetic microsphere 4; self-assembling 5; sensitive 6
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Lou, Z.; Han, H.; Mao, D.; Jiang, Y.; Song, J. Qualitative and Quantitative Detection of PrPSc Based on the Controlled Release Property of Magnetic Microspheres Using Surface Plasmon Resonance (SPR). Nanomaterials 2018, 8, 107.

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