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Nanomaterials 2017, 7(3), 71; doi:10.3390/nano7030071

A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging

1
Environmental Health Sciences Department, Mass Spectrometry Laboratory, IRCCS Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy
2
Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy
3
Oncology Department, Cancer Pharmacology Laboratory, IRCCS Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy
4
Department of Molecular Biotechnology and Health Science, University of Torino, Via Nizza 52, 10126 Torino, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Nicola Cioffi, Rosaria Anna Picca and Francesco Palmisano
Received: 6 February 2017 / Revised: 13 March 2017 / Accepted: 16 March 2017 / Published: 21 March 2017
(This article belongs to the Special Issue Nanomaterials for Mass Spectrometry Applications)
View Full-Text   |   Download PDF [7735 KB, uploaded 21 March 2017]   |  

Abstract

The imaging of drugs inside tissues is pivotal in oncology to assess whether a drug reaches all cells in an adequate enough concentration to eradicate the tumor. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) is one of the most promising imaging techniques that enables the simultaneous visualization of multiple compounds inside tissues. The choice of a suitable matrix constitutes a critical aspect during the development of a MALDI-MSI protocol since the matrix ionization efficiency changes depending on the analyte structure and its physico-chemical properties. The objective of this study is the improvement of the MALDI-MSI technique in the field of pharmacology; developing specifically designed nanostructured surfaces that allow the imaging of different drugs with high sensitivity and reproducibility. Among several nanomaterials, we tested the behavior of gold and titanium nanoparticles, and halloysites and carbon nanotubes as possible matrices. All nanomaterials were firstly screened by co-spotting them with drugs on a MALDI plate, evaluating the drug signal intensity and the signal-to-noise ratio. The best performing matrices were tested on control tumor slices, and were spotted with drugs to check the ion suppression effect of the biological matrix. Finally; the best nanomaterials were employed in a preliminary drug distribution study inside tumors from treated mice. View Full-Text
Keywords: mass spectrometry imaging; MALDI; TiO2; gold nanoparticles; halloysite; cancer imaging mass spectrometry imaging; MALDI; TiO2; gold nanoparticles; halloysite; cancer imaging
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Giordano, S.; Pifferi, V.; Morosi, L.; Morelli, M.; Falciola, L.; Cappelletti, G.; Visentin, S.; Licandro, S.A.; Frapolli, R.; Zucchetti, M.; Pastorelli, R.; Brunelli, L.; D’Incalci, M.; Davoli, E. A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging. Nanomaterials 2017, 7, 71.

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